Nitric oxide (NO) is a reactive radical that performs an essential position in many essential physiological features. NO, an oxidation merchandise of arginine by nitric oxide synthase, is associated in host defense and growth, activation of regulatory proteins and direct covalent conversation with functional biomolecules. Easy, direct and automation-prepared procedures for measuring NO are becoming popular in Investigation and Drug Discovery. Considering that NO is oxidized to nitrite and nitrate, it is frequent practice to quantitate total NO2-/NO3- as a measure for NO stage. BioAssay Systems' QuantiChrom Nitric Oxide Assay Kit is made to correctly measure NO production adhering to reduction of nitrate to nitrite utilizing improved Griess strategy. The method is straightforward and the time necessary for sample pretreatment and assay is decreased to as quick as 30 min. This merchandise is for Investigation Use only and is not intended for diagnostic needs.
Determination of nitric oxide and evaluation of drug effects on its metabolism.
Method: Colorimetric assay (540nm). A spectrophotometer or plate reader is required, but not provided.
Samples: Plasma, serum, urine, tissue/cells and foods. Species: All. Detects 0.6 uM
Procedure: 40 min. Size: 100 tests per kit